Plant Science & Botany

Fundamentals, Methods, and Applications

Editors: Chukwuebuka Egbuna
Jonathan Chinenye Ifemeje, PhD
Stanley Chidi Udedi, PhD
Shashank Kumar, PhD

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This forthcoming volume, Phytochemistry: Fundamentals, Methods, and Applications, plans to cover all areas of phytochemistry. It is being designed to be useful to students in both postgraduate and undergraduate levels and to everyone embarking or doing research in the field of phytochemistry. The book’s scope will embrace several disciplines, such as biological science, biochemistry, pharmacy, food and medicinal chemistry, systematic botany and taxonomy, ethnobotany, conservation biology, plant genetic and metabolomics, evolutionary sciences, and plant pathology. The book will include chapters on methods and modern analytical techniques, including state-of-the-art approaches and computational techniques in the field of phytochemistry.

The volume’s authors are professional specialists in their fields and have been selected carefully to deliver well-structured chapters. The volume aims to be a comprehensive coverage of phytochemistry.

Key features and topics planned for the volume:

• An introduction to phytochemistry, definition, scope and history, phytochemicals, sources, classifications, biosynthesis, etc.
• Phytochemistry in health and disease management
• Recent advances in phytochemical research
• Analytical methods including state-of-the-art modeling techniques and quality indicators
• Computational approaches to phytochemical studies
• Drug discovery and toxicology studies
• Ethnophytochemistry, including ethnobotany, ethnopharmacognosy, ethnoveterinary, and complementary and alternative medicines
• Phytomedicine and nutraceuticals
• Biotechnology approaches
• Marine phytochemistry and plant metabolomics
• Phytochemical biopesticides, including insecticides, fungicides, and herbicides of plant origin
• Environmental influences on phytochemicals
• Phytoremediation chemistry
• Effects of processing methods
• Green tea chemistry
• Wine chemistry
• Biosynthesis of nanoparticles
• And more



1. Introduction to Phytochemistry
Egbuna, C., Ifemeje, J. C., Toskë L. Kryeziu, and Lekë L. Kryeziu
2. Biosynthesis of Phytochemicals
Tijjani Habibu

3. Phytochemistry in Plant Classification: Techniques in Chemotaxonomy
Felix Ifeanyi Nwafor and Ifeoma Celestina Orabueze

4. Emerging Roles of Phytoalexins in Plant Protection: A Prospective Study
Parjanya Kumar Shukla, Amita Verma, Pragya Mishra

5. Phytoalexins Induction and Its Defense Mechanisms against Pathogens
Hanan M. Al-Yousef and Musarat Amina

6. Biological Roles of Phytochemicals
Onyekere Peculiar Feenna, Nwankwo Chioma Abigail, Udodeme Helen Ogechukwu, and Nnamani Daniel Okwudili

7. Phytochemicals as Immunomodulators
Behnaz Aslanipour

8. Phytomedicine: An Emerging Field in Clinical biochemistry
Ekwere, Mercy Robert, Ebong Patrick E., Igile, G.O and Atangwho, I .J.

9. Nutraceuticals and Pharmafoods
Anywar Godwin

10. Plant Metabolomics
Sagar Satish Datir and Rakesh Mohan Jha


11. Extraction Methods and General Techniques Involved in Phytochemical Analysis
Banjo, Temitope Temitayo, Akinduti, Paul Akinniyi and Banjo, and Temitope Oluwabunmi

12. Chromatographical Techniques in Phytochemical Research
Sumera Javad and Shagufta Naz

13. UV/VIS Spectroscopy and HPLC in Phytochemical Analysis
Ade Kujore

14. HPLC and HPTLC as Sophisticated Tools in Phytochemical Analysis
Deepa R. Verma and Rohan V. Gavankar
15. Phytochemical Test Methods
Egbuna, C., Ifemeje, J. C. Maduako, C., Udedi, Stanley, C. and Nwaka, A. C.

16. Analytical Techniques in Elemental Profiling
Andrew G. Mtewa and Annu Amanjot

17. Animal Models in Phytopharmacology
Ahmed A. Adedeji, Mulkah O. Ajagun-Ogunleye, and Marta Vicente-Crespo

18. Toxicological Testing of Plant Products
Monica Neagu and Carolina Constantin

19. Computational Phytochemistry in Drug Discovery: Databases and Tools
Sugumari Vallinayagam, Karthikeyan Rajendran, and Vigneshkumar Sekar

20. Molecular Docking and Applications in ADMET and Drug Metabolism predictions
Umang Shah, Jagat Upadhyay, and Inaxi Parmar

21. Biostatistics in Phytochemical Research
Laurence John Francis J. Gido

22. Phytochemical Analysis of Nigella sativa L. seeds Aqueous Extract by GCMS and FTIR
N. Shaista Jabeen and K. Devi
23. GC-MS analysis and in vitro Anti-cancer Activity of Tectona grandis Bark Extract on Human Breast Cancer Cell Line (MCF-7)
Arul Priya, R., Saravanan, K, and Umarani, B.

24. Phytochemical Studies on Indigenous Vegetables in Okitipupa LGA of Ondo State, Nigeria
Faleyimu, O.I., Solomon, T., and Adebayo, A.J.

25. Stemness Modulation by Phytochemicals to Target Cancer Stem Cells: An in silico study
Prem Prakash Kushwaha and Shashank Kumar

26. Targeting Cancer Cell Carbohydrate Metabolism by Phytochemicals
Swastika Dash, Prem Prakash Kushwaha and Shashank Kumar

About the Authors / Editors:
Editors: Chukwuebuka Egbuna
Research Biochemist, Department of Biochemistry, Faculty of Natural Sciences, Chukwuemeka Odumegwu Ojukwu University, Nigeria

Chukwuebuka Egbuna is a chartered chemist, a chemical analyst, and an academic researcher. He is a member of the Institute of Chattered Chemists of Nigeria (ICCON), the Nigerian Society of Biochemistry and Molecular Biology (NSBMB), and the Society of Quality Assurance (SQA) (USA). He has been engaged in a number of roles at New Divine Favor Pharmaceutical Industry Limited, Akuzor Nkpor, Anambra State, Nigeria, and Chukwuemeka Odumegwu Ojukwu University (COOU), Nigeria. He has collaboratively worked and published quite a number of research articles in the area of phytochemistry and its applications. He is a reviewer and editorial board member of various journals, with his most recent role as a website administrator for the Tropical Journal of Applied Natural Sciences (TJANS), a journal of the faculty of Natural Sciences, COOU. His primary research interests are in phytochemistry, food and medicinal chemistry, analytical chemistry, and nutrition and toxicology. He obtained his BSc and MSc degrees in biochemistry at Chukwuemeka Odumegwu Ojukwu University.

Jonathan Chinenye Ifemeje, PhD
Senior Lecturer, Department of Biochemistry, Faculty of Natural Sciences, Chukwuemeka Odumegwu Ojukwu University, Nigeria

Jonathan Chinenye Ifemeje, PhD, is a Senior Lecturer in the Department of Biochemistry, Faculty of Natural Sciences, Chukwuemeka Odumegwu Ojukwu University, Nigeria. He has to his credits over 40 publications and has served as an external examiner for various institutions. He is the Managing Editor of the Tropical Journal of Applied Natural Sciences and is currently serving as a reviewer and an editorial board member for various journals. He has worked extensively in the area of phytochemistry, nutrition, and toxicology. He is a member of various institutes, including the Institute of Chattered Chemists of Nigeria (ICCON), the Nigerian Society of Biochemistry and Molecular Biology (NSBMB), and Society of Quality Assurance (SQA). He obtained his PhD in applied biochemistry from Nnamdi Azikiwe University, Awka, Nigeria, and his MSc degree in nutrition and toxicology from the University of Port-Harcourt, Nigeria.

Stanley Chidi Udedi, PhD
Full Professor of Biochemistry, Department of Applied Biochemistry, Nnamdi Azikiwe University, (UNIZIK), Awka, Nigeria

Stanley Chidi Udedi, PhD, is a Full Professor of Biochemistry at the Department of Applied Biochemistry, Nnamdi Azikiwe University, (UNIZIK), Awka, Nigeria. He has taught in various institutions, including Madonna University Okija, Nigeria, and Anambra State University, which is now Chukwuemeka Odumegwu Ojukwu University, Nigeria. He is currently the Dean of Student Affairs at UNIZIK. He has worked extensively in the field of phytochemistry and food and medicinal chemistry and has supervised many MSc and PhD students. He is well published with over 50 publications, which has attracted over 170 citations. He is well traveled and has attended a series of workshops and conferences locally and internationally. He is a member of various professional institutes, which includes the Institute of Chattered Chemists of Nigeria (ICCON), the Nigerian Society of Biochemistry and Molecular Biology (NSBMB), the Nutrition Society of Nigeria, and others. He obtained his PhD and MSc degrees from UNIZIK, Nigeria.

Shashank Kumar, PhD
Assistant Professor, Center for Biochemistry and Microbial Sciences, Central University of Punjab, Bathinda, India

Shashank Kumar, PhD, is working as Assistant Professor at the Center for Biochemistry and Microbial Sciences, Central University of Punjab, Bathinda, India. He obtained his BSc, MSc, and PhD degrees in biochemistry from the Department of Biochemistry, University of Allahabad, Allahabad, India. He has worked as a Postdoctoral Fellow at the Department of Biochemistry, King George’s Medical University, Lucknow, India. Dr. Kumar has about 60 published scientific papers, reviews, editorial articles, and book chapters in various national and international peer-reviewed journals and has been cited more than 1,200 times. He has edited several books including Concepts in Cell Signaling and Carbohydrate Metabolism: Theory and Practical Approach, among others. He has expertise in the areas of free radical biology, cancer biology, characterization of plant products, xenobiotic metabolism, and microbiology. He is familiar with many biochemical techniques such as spectrophotometry, ELISA, electrophoresis, PCR, RT-PCR, flow cytometry, TLC, HPLC, LC-MS, cell culture, and microbiological techniques. He has presented his research findings at more than 25 national and international conferences and has attended about 30 workshops at many major universities and medical colleges throughout the India. Dr. Kumar is a life-time member of the Italo-Latin American Society of Ethnomedicine, and the Indian Sciences Congress Association, India, and a member of the Asian Council of Science Editors, Dubai, UAE and Publication Integrity & Ethics, London. He has been awarded junior, senior, and research associate fellowships formulated and funded by various Indian agencies, such as the Indian Council of Medical Research, University Grants Commission, and the Council of Scientific and Industrial Research India. Dr. Kumar’s laboratory has been funded by the University Grant Commission, India, and the Department of Science and Technology, India, for working on the effect of various phytochemicals on cancer cell signaling pathway inhibition.

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